Designing our future
Part of the Technical University of Denmark and DTU Bioengineering, the Digital Biotechnology Lab is a junior research group within the Tropical Pharmacology Lab. We are passionate about harnessing the power of modern technology to innovate the biotechnological landscape and develop real world solutions for the benefit of society. We focus on leveraging the power of data science and automation to discover and understand protein-based binders, such as antibodies, nanobodies, and DARPins. Specifically, we are interested in (1) generating large scale antibody-antigen datasets via phage/yeast display together with next-generation sequencing, which can be used for machine learning, and (2) in silico approaches for the de novo design or affinity maturation of binders. Importantly, this not only allows us to pursue fundamental questions, such as unravelling the core rule set that dictates binder-target interactions, but also translational endeavours, such as discovering cost-effective therapeutics against infectious and neglected tropical diseases (e.g., snakebite envenomings).
FOCUS AREAS
Fundamental
What dictates cross-reactivity of antibodies? Many variables are at play in antibody-target interactions. At the Digital Biotechnology Lab we are trying to understand which ones matter the most, and whether we can use that knowledge to discover more cross-reactive binders. For that purpose, we generate large, multifaceted datasets and employ structural modelling (e.g., AlphaFold2) and de novo design (e.g., RFDesign), as well as mapping the antibody-target interface. With the insights we gain and the data generated in the process we train machine learning algorithms to aid binder discovery and development.
Translational
Monoclonal antibodies have been developed into ‘blockbuster drugs’ and are predicted to reach a market value of 300 billion USD in 2025. Yet, whilst their high specificity and affinity for target antigens make them highly beneficial for many indications, different tools are required when multiple targets need to be neutralised. This is the case with animal venoms that comprise multitudes of similar and dissimilar toxins, but also for mutating diseases, such as cancer and infectious diseases. In such cases, it is beneficial, if not essential, to use broadly-neutralising antibodies that are able to target multiple similar antigens, do not easily lose their efficacy when faced with rapid target mutation, and are easy to manufacture. Thus, at the Digital Biotechnology Lab we use display technologies as well as generative ML to discover broadly-neutralising antibodies and antibody alternatives such as DARPins, nanobodies, and minibinders against animal and plant toxins.
Entrepreneurship
At the Digital Biotechnology Lab, we leverage scientific advances to solve the world’s problems. For this reason, significant emphasis is put on evaluating new ideas and findings in an entrepreneurial context. To date, two commercial projects, Sweathut Therapeutics and Immunite, have received soft funding and are being development further.
THE DIGITAL BIOTECHNOLOGY LAB TEAM
Get to Know Us
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Alumni
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PEER REVIEWED PUBLICATIONS
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Christoffer V. Sørensen, Line Ledsgaard, Helen H. K. Wildenauer, Camilla H. Dahl, Tasja W. Ebersole, Markus-Frederik Bohn, Anne Ljungars, Timothy P. Jenkins, & Andreas H. Laustsen. Cross-reactivity trends when selecting scFv antibodies against snake toxins using a phage display-based cross-panning strategy. SciRep (2023).
LKonstantinos Kalogeropoulos, Markus-Frederik Bohn, David E. Jenkins, Jann Ledergerber, Christoffer V. Sørensen, Nils Hofmann, Jack Wade, Thomas Fryer, Giang Thi Tuyet Nguyen, Ullrich auf dem Keller, Andreas H. Laustsen, Timothy P. Jenkins. A Comparative Study of Protein Structure Prediction Tools for Challenging Targets: Snake Venom Toxins. bioRxiv (2023).
William M. Laprade, Keirah E. Bartlett, Charlotte R. Christensen, Taline D. Kazandjian, Rohit N. Patel, Edouard Crittenden, Charlotte A. Dawson, Marjan Mansourvar, Darian S. Wolff, Thomas J. Fryer, Andreas H. Laustsen, Nicholas R. Casewell, Jose Maria Gutierrez, Steven R. Hall, Timothy P. Jenkins. Machine-learning guided Venom Induced Dermonecrosis Analysis tooL: VIDAL. bioRxiv (2023).
Monica L. Fernández-Quintero, Anne Ljungars, Franz Waibl, Victor Greiff, Jan Terje Andersen, Torelif T. Gjølberg, Timothy P. Jenkins, Bjørn Gunnar Voldborg, Lise Marie Grav, Sandeep Kumar, Guy Georges, Hubert Kettenberger, Klaus R. Liedl, Peter M. Tessier, John McCafferty & Andreas H. Laustsen. Assessing developability early in the discovery process for novel biologics. mAbs (2023).
Line Ledsgaard, Jack Wade, Timothy P. Jenkins, Kim Boddum, Irina Oganesyan, Julian A. Harrison, Pedro Villar, Rachael A. Leah, Renato Zenobi, Sanne Schoffelen, Bjørn Voldborg, Anne Ljungars, John McCafferty, Bruno Lomonte, José M. Gutiérrez, Andreas H. Laustsen & Aneesh Karatt-Vellatt. Discovery and optimization of a broadly-neutralizing human monoclonal antibody against long-chain α-neurotoxins from snakes. Nat Commun (2023).
Marina Papaiakovou, Natalia Fraija-Fernández, Katherine James, Andrew G. Briscoe, Andie Hall, Timothy P. Jenkins, Julia Dunn, Bruno Levecke, Zeleke Mekonnen, Piet Cools, Stephen R. Doyle, Cinzia Cantacessi, DTJ Littlewood. Evaluation of genome skimming to detect and characterise human and livestock helminths. Parasitol. Int. (2023).
Timothy P. Jenkins, Nicolas Lopez Carranza, Amy Bray, Karim Beguir, Andreas H. Laustsen. Can hackathons unlock a new talent pool from the developing world? Nat Biotechnol (2022).
Timothy P. Jenkins, William Michael Laprade, Andrés Sánchez, Tulika Tulika, Carol O’Brien, Christoffer V. Sørensen, Trenton K. Stewart, Thomas Fryer, Andreas H. Laustsen, José María Gutiérrez. AHA: AI-guided tool for the quantification of venom-induced haemorrhage in mice. Front. Trop. Dis (2022).
Andreas H. Laustsen, Bengt H. Gless, Timothy P. Jenkins, Maria Meyhoff-Madsen, Johanna Bjärtun, Andreas S. Munk, Saioa Oscoz, Julián Fernández, José María Gutiérrez, Bruno Lomonte, Brian Lohse. “In vivo neutralization of Myotoxin II, a phospholipase A2 homolog from Bothrops asper venom, using peptides discovered via phage display technology”. ACS Omega (2022).
Wei-qiao Rao, Konstantinos Kalogeropoulos, Morten E. Allentoft, Shyam Gopalakrishnan, Weining Zhao, Christopher T. Workman, Cecilie Knudsen, Belén Jiménez-Mena, Lorenzo Seneci, Mahsa Mousavi-Derazmahalleh, Timothy P. Jenkins, Si-qi Liu, Andreas H. Laustsen “The rise of genomics in snake venom research: recent advances and future perspectives”. GigaScience (2022), 11.
Esperanza Rivera-de-Torre, Charlotte Rimbault, Anna D. Jensen, Christoffer Sorensen, Timothy P. Jenkins, Natalie Saez, Yoan Duhoo, Celeste Menuet, Lars Ellgaard, Andreas H. Laustsen. “Strategies for heterologous expression and purification of animal venom toxins”. Frontiers in Bioengineering and Biotechnology (2022), 9.
Timothy P. Jenkins, Shirin Ahmadi, Matyas Bittenbinder, Trenton Keith Stewart, Dilber E. Akgun, Melissa Hale, Nafiseh N. Nasrabadi, Darian S. Wolff, Freek J. Vonk, Jeroen Kool, and Andreas H. Laustsen. “Terrestrial venomous animals and the envenomings they cause in the Middle East and North Africa”. PLoS NTD (2021), 15 (12).
Nicolai Rügen, Timothy P. Jenkins, Natalie Wielsch, Heiko Vogel, Benjamin-Florian Hempel, Roderich D. Süssmuth, Stuart Ainsworth, Alejandro Cabezas-Cruz, Andreas Vilcinskas, and Miray Tonk. "Hexapod Assassins’ Potion: Venom Composition and Bioactivity from the Eurasian Assassin Bug Rhynocoris iracundus." Biomedicines 9, no. 7 (2021): 819.
Timothy P. Jenkins, David I. Pritchard, Gary Telford, Radu Tanasescu, Cris S. Constantinescu, and Cinzia Cantacessi. “Longitudinal changes in the gut microbiome of human volunteers with remitting-relapsing multiple sclerosis following experimental infection with the hookworm, Necator americanus”. BMC Biology (2021), 19, 74.
Andreas H. Laustsen, Victor Greiff, Aneesh Karatt-Vellatt, Serge Muyldermans, Timothy P. Jenkins. “Animal immunization, in vitro display technologies, and machine learning for antibody discovery”. Trends in Biotechnology (2021).
Timothy P. Jenkins and Andreas H. Laustsen. “Cost of manufacturing for recombinant snakebite antivenoms”. Frontiers in Bioengineering and Biotechnology (2020), 8.
Timothy P. Jenkins, Kamille E. Krause, Carina Skaarup, Mikael Engmark, Nicholas Casewell, Robert Harrison, Stuart Ainsworth, Bruno Lomonte, Julián Fernández, José María Gutiérrez, Andreas H. Laustsen, and Ole Lund. “An interactive database for the investigation of high-density peptide microarray guided interaction patterns and antivenom cross-reactivity”. PLoS Neglected Tropical Diseases, (2020), 14, 6.
Radu Tanasescu, Christopher R. Tench, Cris S. Constantinescu, Gary Telford, Sonika Singh, Nanci Frakich, David Onion, Dorothee P. Auer, Bruno Gran, Nikos Evangelou, Yasser Falah, Colin Renshaw, Cinzia Cantacessi, Timothy P. Jenkins and David I. Pritchard. “Hookworm Treatment for Relapsing Multiple Sclerosis: A Randomized Double-Blinded Placebo-Controlled Trial”. JAMA Neurolology, (2020), published online.
2024
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Timothy Patrick Jenkins, Norbert Ács, Emma Wenzel Horsted, Abbie Williams, Ágnes Duzs, Ioanna Chatzigiannidou, Michael Pichler, Tiia Kittilä, Laura Peachey, Lone Gram, Nuria Canibe, Andreas Hougaard Laustsen, Susanne Brix, and Sandra Wingaard Thrane. "Protecting the piglet gut microbiota against ETEC-mediated post-weaning diarrhea using specific binding proteins." npj Biofilms and Microbiomes (accepted).
Keirah E Bartlett, Steven R Hall, Sean A Rasmussen, Edouard Crittenden, Charlotte A Dawson, Laura-Oana Albulescu, William Laprade, Robert A Harrison, Anthony J Saviola, Cassandra M Modahl, Timothy P Jenkins, Mark C Wilkinson, José María Gutiérrez, Nicholas R Casewell. "Dermonecrosis caused by spitting cobra snakebite results from toxin potentiation and is prevented by the repurposed drug varespladib." PNAS (accepted).
Christoffer V Sørensen, Nils Hofmann, Puneet Rawat, Frederik V Sørensen, Anne Ljungars, Victor Greiff, Andreas H Laustsen, Timothy P Jenkins. ”ExpoSeq: simplified analysis of high-throughput sequencing data from antibody discovery campaigns”. Bioinformatics Advances (2024).
Katie Bull, Gareth Davies, Timothy P. Jenkins, and Laura Peachey. "The faecal microbiome of Exmoor ponies shows step‐wise compositional changes with increasing levels of management by humans." Equine Veterinary Journal (2023).
Kevin Eloff, Konstantinos Kalogeropoulos, Oliver Morell, Amandla Mabona, Jakob Berg Jespersen, Wesley Williams, Sam van Beljouw, Marcin Skwark, Andreas Hougaard Laustsen, Stan J. J. Brouns, Anne Ljungars, Erwin M. Schoof, Jeroen Van Goey, Ulrich auf dem Keller, Karim Beguir, Nicolas Lopez Carranza, and Timothy P. Jenkins. De novo peptide sequencing with InstaNovo: Accurate, database-free peptide identification for large scale proteomics experiments. bioRxiv (2023).
Andreas V. Madsen, Oscar Mejias-Gomez, Kerstin Skovgaard, Lasse E. Pedersen, J. Preben Morth, Peter Kristensen, Timothy P. Jenkins, & Steffen Goletz. Characterizing antibody binding interfaces: a computational analysis of 1833 experimentally determined antibody:antigen 3D structures. Computational and Structural Biotechnology Journal (2023).
Oscar Mejias-Gomez, Andreas V. Madsen, Kerstin Skovgaard, Lasse E. Pedersen, J. Preben Morth, Timothy P. Jenkins, Peter Kristensen, and Steffen Goletz. A Window into the Human Immune System: Comprehensive Characterization of the Complexity of Antibody Complementary Determining Regions in functional antibodies. mAbs (2023).
2023
2023
GRANTS & PRIZES
Otto Monsted Travel Grant (TPJ)
USD 1,000
NNF Data Science
USD 208,000
NNF Data Science
USD 208,000
AIDA Award (MabSilico)
USD 170,000
InnovationFund Industrial PhD
USD 207,000
NNF Project Grants in Bioscience
USD 436.768
ERC PoC - Immunite
USD 169,000
Otto Monsted Travel Grant (CRC)
USD 1,000
InnoExplorer Immunite
USD 40,000
DTU PoC - Immunite
USD 68,520
ERC PoC - CAT
USD 169,000
Alliance PhD
USD 210,000
DTU Discovery grant - Immunite
USD 22,250
Novo Scholarship - Darian Wolff
USD 8,500
H.C. Ørsted Fellowship extension
USD 62,500
InnoExplorer - Sweathut Therapeutics
USD 250,000
Hørslev-Fonden
USD 30,000
Otto Monsted - ‘Bright Idea Award’
USD 40,000
DTU Discovery grant - Sweathut Therapeutics
USD 22,250
Brødrene Hartmanns Fond
USD 13,200
H.C. Ørsted Postdoctoral Fellowship
USD 177,300
POPULAR SCIENCE
2023
ScienceNordic
Andreas Rygaard, Timothy P. Jenkins, James Love, Søren Bertelsen.
Here is how an algorithm is baking optimal brownies and discovering drugs
2023
Videnskab
Andreas Rygaard, AnneLjungars, Thomas Fryer, Timothy P. Jenkins.
Sådan kan en algoritme bruges til at bage de bedste brownies og udvikle lægemidler
2023
ScienceNordic
Andreas Rygaard, AnneLjungars, Thomas Fryer, Timothy P. Jenkins.
These magic bullet-proteins are revolutionizing modern medicine
2023
Videnskab
Andreas Rygaard, AnneLjungars, Thomas Fryer, Timothy P. Jenkins.
2023
ScienceNordic
Andreas Rygaard, AnneLjungars, Thomas Fryer, Timothy P. Jenkins.
How a Nobel prize-winning technology is speeding up evolution
2023
Videnskab
Andreas Rygaard, AnneLjungars, Thomas Fryer, Timothy P. Jenkins.
Nobelprisvindende teknologi sætter turbo på udviklingen af lægemidler
2022
ScienceNordic
Timothy P. Jenkins, Lorenzo Seneci, Christoffer V. Sørensen, Charlotte R. Christensen.
Introducing the poison that inspired Van Gogh and almost killed James Bond: Digoxin
2022
Videnskab
Timothy P. Jenkins, Lorenzo Seneci, Christoffer V. Sørensen, Charlotte R. Christensen.
Dette giftstof tog næsten livet af James Bond, inspirerede Van Gogh – og bruges i dag som medicin
2022
ScienceNordic
Lorenzo Seneci, Timothy P. Jenkins, Shirin Ahmadi, Christoffer V. Sørensen.
2022
Videnskab
Lorenzo Seneci, Timothy P. Jenkins, Shirin Ahmadi, Christoffer V. Sørensen.
“Hallucinationer og lægemidler: Psykedeliske svampes mange funktioner”
2022
ScienceNordic
Lorenzo Seneci, Timothy P. Jenkins, Shirin Ahmadi, Christoffer V. Sørensen.
“Cell death and vomiting: This happens in your body, if you eat poisonous mushrooms”
2022
Videnskab
Lorenzo Seneci, Timothy P. Jenkins, Shirin Ahmadi, Emilie Henriksen, Christoffer V. Sørensen.
“Celledød og opkast: Det sker i kroppen, hvis du spiser giftige svampe”
2021
Videnskab
Lorenzo Seneci, Timothy P. Jenkins, Christoffer V. Sørensen.
“Ekstremt stor forskel på slangernes gift: Ikke to bid er ens”.
2021
ScienceNordic
Lorenzo Seneci, Timothy P. Jenkins, Christoffer V. Sørensen.
“Extreme variation in snake venom: no two bites are the same”.
2020
ScienceNordic
Anna D. Jensen, Timothy P. Jenkins, Thomas Fryer, Andreas H. Laustsen.
2020
Videnskab
Anna D. Jensen, Timothy P. Jenkins, Thomas Fryer, Andreas H. Laustsen.
“Nanobodies: Gemmer kamelens immunforsvar på en mindre og bedre version af antistoffer?”
2020
The Conversation
Andreas H. Laustsen, Line Ledsgard, and Timothy P. Jenkins
“Finally, snakebite is getting more attention as a tropical health issue”
RECENT MEDIA
1 JUN 2023 BioTechniques
JOIN US
At the Digital Biotechnology Lab we are always excited to hear from passionate scientists and students who have an interest in joining our research group. We welcome everyone across ages, nationalities, cultures, genders, and experience levels.
If you would like to join our lab, please follow these guidelines:
Future of Drug Discovery talkshow
FOR ASPIRING INTERNS AND VOLUNTEERS
If you are interested in joining our group as an intern or volunteer and would like to conduct research and learn new techniques, please send the following to tpaje@dtu.dk:
Letter of motivation (max 1 page)
CV
Grade transcripts
Be aware that funding is generally not available to support travel or accommodation expenses for students coming from abroad. Exceptions generally only apply to students/graduates from close collaborators.
FOR BSC AND MSC STUDENTS
If you are interested in joining our group during your bachelor or master studies and would like to conduct a research project (bachelor, master, or special project), please send the following to tpaje@dtu.dk:
Letter of motivation (max 1 page)
CV
Grade transcripts
Be aware that no funding is available to support travel or accommodation expenses for students coming from abroad.
FOR ASPIRING PHD STUDENTS
When available, fully funded PhD positions are posted on https://www.dtu.dk/english/about/job-and-career/vacant-positions. These positions will also be advertised on Twitter on @TimothyPJenkins. If no open positions are listed here, none are available.
If you are interested in joining as a PhD student within our group and have secured your own funding, please send the following to tpaje@dtu.dk:
Letter of motivation (max 1 page)
CV
Grade transcripts
FOR POSTDOCS AND POSTDOCTORAL FELLOWS
When available, fully funded postdoc positions are posted on https://www.dtu.dk/english/about/job-and-career/vacant-positions. These positions will also be advertised on Twitter on @TimothyPJenkins. If no open positions are listed here, none are available!
If you are interested in working as a postdoc within our group and have either already obtained or intend to obtain your own funding by preparing a fellowship application to a funding agency, please send the following to tpaje@dtu.dk:
Letter of motivation (max 1 page)
CV